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high capacity complementary deoxyribonucleic acid cdna reverse transcription rt kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher high capacity complementary deoxyribonucleic acid cdna reverse transcription rt kit
    High Capacity Complementary Deoxyribonucleic Acid Cdna Reverse Transcription Rt Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+capacity+rt+kit/Deoxyribonucleic+acid/pm42019360-59-0-12
    Average 99 stars, based on 1 article reviews
    high capacity complementary deoxyribonucleic acid cdna reverse transcription rt kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Quantitative RT-PCR:

    Article Title: Preferential Binding of Hot Spot Mutant p53 Proteins to Supercoiled DNA In Vitro and in Cells
    Article Snippet: When appropriate, 500 ng of the p53 expression vector or empty vector pCDNA3.1 were used. .. In the stress condition cells were exposed to 0.1 μM doxorubicin for 16 h . For qRT-PCR analysis, total RNA was isolated by applying NucleoSpin RNA II (MachereyNagel - according to the manufacturés instruction) and 2 μg of RNA were reverse transcribed by the High Capacity RT kit (Applied Biosystems - according to manufacturés protocol). .. PCR was performed using the Power SYBR Green PCR Master Mix (Applied Biosystems) and EvaGreen (Solis Biodyne) in the standard program (15 min 95°C; 15 s 95°C, 30 s 60°C, 20 s 72°C, 10 s 74°C; 45 cycles) running in an RotorGene 6000 (Corbett Research) and 7900HT Fast Real-Time PCR system (Applied Biosystems).

    Isolation:

    Article Title: Preferential Binding of Hot Spot Mutant p53 Proteins to Supercoiled DNA In Vitro and in Cells
    Article Snippet: When appropriate, 500 ng of the p53 expression vector or empty vector pCDNA3.1 were used. .. In the stress condition cells were exposed to 0.1 μM doxorubicin for 16 h . For qRT-PCR analysis, total RNA was isolated by applying NucleoSpin RNA II (MachereyNagel - according to the manufacturés instruction) and 2 μg of RNA were reverse transcribed by the High Capacity RT kit (Applied Biosystems - according to manufacturés protocol). .. PCR was performed using the Power SYBR Green PCR Master Mix (Applied Biosystems) and EvaGreen (Solis Biodyne) in the standard program (15 min 95°C; 15 s 95°C, 30 s 60°C, 20 s 72°C, 10 s 74°C; 45 cycles) running in an RotorGene 6000 (Corbett Research) and 7900HT Fast Real-Time PCR system (Applied Biosystems).

    Reverse Transcription:

    Article Title: Preferential Binding of Hot Spot Mutant p53 Proteins to Supercoiled DNA In Vitro and in Cells
    Article Snippet: When appropriate, 500 ng of the p53 expression vector or empty vector pCDNA3.1 were used. .. In the stress condition cells were exposed to 0.1 μM doxorubicin for 16 h . For qRT-PCR analysis, total RNA was isolated by applying NucleoSpin RNA II (MachereyNagel - according to the manufacturés instruction) and 2 μg of RNA were reverse transcribed by the High Capacity RT kit (Applied Biosystems - according to manufacturés protocol). .. PCR was performed using the Power SYBR Green PCR Master Mix (Applied Biosystems) and EvaGreen (Solis Biodyne) in the standard program (15 min 95°C; 15 s 95°C, 30 s 60°C, 20 s 72°C, 10 s 74°C; 45 cycles) running in an RotorGene 6000 (Corbett Research) and 7900HT Fast Real-Time PCR system (Applied Biosystems).

    Article Title: Transplacental arsenic exposure produced 5-methylcytosine methylation changes and aberrant microRNA expressions in livers of male fetal mice
    Article Snippet: .. Total RNA was reverse transcribed with Multiscript reverse transcriptase using a High Capacity RT kit from Applied Biosystems (Applied Biosystems, Foster City, CA). ..

    Article Title: Genetic Activation of Nrf2 Protects against Fasting-Induced Oxidative Stress in Livers of Mice
    Article Snippet: RNA was dissolved in diethyl pyrocarbonate-treated deionized water, and RNA concentration was determined with a NanoDrop Spetrophotometer ND-1000 (Thermo Scientific, Wilmington, DE). .. Total RNA was reverse-transcribed into first-strand cDNA using multiscript reverse transcriptase from a High Capacity RT kit (Applied Biosystems, Foster City, CA) according to the manufacturer’s protocol. .. Messenger RNA of genes of interest was determined with quantitative real time-PCR performed on an Applied Biosystems Prism 7900HT sequence detection system.

    Article Title: Overexpression of Nrf2 Protects against Microcystin-Induced Hepatotoxicity in Mice
    Article Snippet: .. Total RNA was reverse-transcribed into cDNA by a High Capacity RT Kit (Applied Biosystems, Foster City, CA), and amplified with Power SYBR Green PCR Master Mix in a 7900HT PCR System (Applied Biosystems, Foster City, CA). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Single cell RNA sequencing of human FAPs reveals different functional stages in Duchenne muscular dystrophy.
    Article Snippet: Total RNA was extracted from cell pellets using PureLink RNA Mini Kit (Invitrogen) and quantified using nanodrop ND-100 spectrophotometer (Nanodrop Technologies, Wilmington, DE, United States). .. Five hundred ng of total RNA was retrotranscribed using the High Capacity RT Kit (Applied Biosystems, Foster City, CA) following manufacturer’s instructions. qPCR was performed by triplicate using the Fast Advanced Master Mix and a 7900 HT Fast Real-Time PCR system (ThermoFisher). .. The mRNA-specific Taqman probes (Applied Biosystems) were used for the different genes.

    other:

    Article Title: Purification of mitochondria from skeletal muscle tissue for transcriptomic analyses reveals localization of nuclear‐encoded noncoding RNAs
    Article Snippet: 400 pg RNA from the RNase‐treated mitochondria samples was reverse‐transcribed to first‐strand cDNA in a 20 μL reaction along with no‐template and no‐amplification controls (Applied Biosystems High Capacity RT kit #4368814).

    Article Title: Purification of mitochondria from skeletal muscle tissue for transcriptomic analyses reveals localization of nuclear‐encoded noncoding RNAs
    Article Snippet: Equal volumes of isolated RNA from the RNase‐treated mitochondria samples (to account for differences in total RNA yield between samples due to RNase treatment) were reverse‐transcribed to first‐strand cDNA in a 20 μL reaction along with no‐template and no‐RTase controls (Applied Biosystems High Capacity RT kit #4368814).

    Amplification:

    Article Title: Overexpression of Nrf2 Protects against Microcystin-Induced Hepatotoxicity in Mice
    Article Snippet: .. Total RNA was reverse-transcribed into cDNA by a High Capacity RT Kit (Applied Biosystems, Foster City, CA), and amplified with Power SYBR Green PCR Master Mix in a 7900HT PCR System (Applied Biosystems, Foster City, CA). ..

    SYBR Green Assay:

    Article Title: Overexpression of Nrf2 Protects against Microcystin-Induced Hepatotoxicity in Mice
    Article Snippet: .. Total RNA was reverse-transcribed into cDNA by a High Capacity RT Kit (Applied Biosystems, Foster City, CA), and amplified with Power SYBR Green PCR Master Mix in a 7900HT PCR System (Applied Biosystems, Foster City, CA). ..

    Polymerase Chain Reaction:

    Article Title: Overexpression of Nrf2 Protects against Microcystin-Induced Hepatotoxicity in Mice
    Article Snippet: .. Total RNA was reverse-transcribed into cDNA by a High Capacity RT Kit (Applied Biosystems, Foster City, CA), and amplified with Power SYBR Green PCR Master Mix in a 7900HT PCR System (Applied Biosystems, Foster City, CA). ..



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